Isolation and identification of mammalian orthoreovirus type 3 from a Korean roe deer (Capreolus pygargus)

Mammalian reovirus (MRV) causes respiratory and intestinal disease in mammals. Although MRV isolates have been reported to circulate several animals, there are no reports on Korean from wildlife. We investigated the biological molecular characteristics of based nucleotide sequence segment 1 gene. In total, 144 swabs wild animals were prepared for virus isolation. Based isolation with specific cytopathic effects, indirect fluorescence assays, electron microscopy, reverse transcription-polymerase chain reaction, only one isolate was confirmed be a roe deer (Capreolus pygargus). The exhibited hemagglutination activity level 16 units pig erythrocytes had maximum viral titer 105.7 50% tissue culture infectious dose (TCID50)/mL Vero cells at 5 days after inoculation. amino-acid sequences partial S1 MReo2045 determined compared those other strains. similar MRV-3 most (96.1%) T3/Bat/Germany/342/08 strain, which isolated Germany 2008. will useful as an antigen sero-epidemiological studies developing diagnostic tools. Keywords: reovirus, isolation, identification, genetic characterization,